牛奶中大肠杆菌的聚合酶链反应-酶联免疫吸附测定检测
PCR-ELISA detection of Escherichia coli in milk.
作者信息
Daly P, Collier T, Doyle S
机构信息
Department of Biology, National University of Ireland, Maynooth, Kildare, Ireland.
出版信息
Lett Appl Microbiol. 2002;34(3):222-6. doi: 10.1046/j.1472-765x.2002.01074.x.
AIMS
The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk.
METHODS AND RESULTS
Robust and expeditious DNA extraction and PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled amplicons to facilitate optimal detection of E. coli DNA.
CONCLUSIONS
It was found that 5 E. coli colony-forming units (cfu) could be detected per PCR reaction using the PCR-ELISA system, equating to a sensitivity of detection of 100 E. coli cfu ml(-1) pasteurized milk.
SIGNIFICANCE AND IMPACT OF THE STUDY
This approach should facilitate evaluation of milk contamination and enable rapid detection of E. coli mastitis, leading to correct deployment of relevant antibiotic therapy and improved animal welfare.
目的
本研究的目的是开发一种可靠的分子方法用于检测牛奶中的大肠杆菌。
方法与结果
使用酶联免疫吸附测定（ELISA）检测生物素标记的扩增子，对稳健且快速的DNA提取和PCR技术进行了评估，以促进对大肠杆菌DNA的最佳检测。
结论
发现使用PCR-ELISA系统每个PCR反应可检测到5个大肠杆菌菌落形成单位（cfu），相当于检测巴氏杀菌牛奶中100 cfu ml⁻¹大肠杆菌的灵敏度。
研究的意义和影响
这种方法应有助于评估牛奶污染情况，并能够快速检测大肠杆菌性乳腺炎，从而正确部署相关抗生素治疗并改善动物福利。
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