与替代方法相比，欧盟饮用水指令中总大肠菌群和大肠杆菌计数的参考方法
EU Drinking Water Directive reference methods for enumeration of total coliforms and Escherichia coli compared with alternative methods.
作者信息
Schets F M, Nobel P J, Strating S, Mooijman K A, Engels G B, Brouwer A
机构信息
National Institute for Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection, Bilthoven, The Netherlands.
出版信息
Lett Appl Microbiol. 2002;34(3):227-31. doi: 10.1046/j.1472-765x.2002.01075.x.
AIMS
The reference methods for enumeration of total coliforms and Escherichia coli as stated in the European Drinking Water Directive were compared with alternative methods.
METHODS AND RESULTS
Laboratories used the reference method on Lactose TTC agar (LTTC), the Colilert/18 system, Laurysulphate Agar (LSA), Chromocult Coliform Agar and the E. coli Direct Plating (DP) method. They enumerated more total coliforms on LTTC than on LSA.
CONCLUSIONS
LTTC is suitable for analysis of very clean water samples only, due to heavy background growth. Colilert/18 is a good alternative but it enumerates a broader group of total coliforms, resulting in higher counts. The DP method appeared to be the best choice for enumeration of E. coli because Colilert/18 produces lower counts and false-negative results.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study shows the limitations of the EU reference method on LTTC due to lack of selectivity and suggests alternative methods for the enumeration of total coliforms and E. coli.
目的
将欧洲饮用水指令中规定的总大肠菌群和大肠杆菌计数的参考方法与其他替代方法进行比较。
方法与结果
各实验室使用了乳糖TTC琼脂（LTTC）参考方法、Colilert/18系统、月桂基硫酸盐琼脂（LSA）、显色培养基大肠菌群琼脂和大肠杆菌直接平板接种（DP）法。他们在LTTC上计数的总大肠菌群比在LSA上更多。
结论
由于背景生长旺盛，LTTC仅适用于分析非常清洁的水样。Colilert/18是一个很好的替代方法，但它计数的总大肠菌群种类更广泛，导致计数更高。DP法似乎是大肠杆菌计数的最佳选择，因为Colilert/18计数较低且会产生假阴性结果。
研究的意义和影响
本研究显示了欧盟LTTC参考方法因缺乏选择性而存在的局限性，并提出了总大肠菌群和大肠杆菌计数的替代方法。
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