Bernasconi C, Volponi G, Bonadonna L
European Commission, Joint Research Center, Institute for Environment and Sustainability, Via E. Fermi 1, 21020 Ispra (Varese), Italy.
Water Sci Technol. 2006;54(3):141-5. doi: 10.2166/wst.2006.460.
The European Drinking Water Directive defines reference methods for the enumeration of microbiological parameters in drinking water. The method to be used for Escherichia coli and coliforms is the membrane filtration technique on Lactose TTC agar with Tergitol 7. Many technical drawbacks of the procedure, as well as its limitations regarding the recent taxonomy of coliforms, make it necessary to evaluate alternative methods. Two alternative assays, a chromogenic media (m-ColiBlu24) and a defined substrate technology-DST test (Colilert 18/Quanty Tray) were compared with the ISO standard with attention to the phenotypic characteristic of the isolates. Results showed that the ISO method failed to detect an important percentage of coliforms and E. coli while m-ColiBlu24 and Colilert 18 provided results in a shorter time allowing the simultaneous detection of E. coli and coliforms with no further confirmation steps.
《欧洲饮用水指令》定义了饮用水中微生物参数计数的参考方法。用于检测大肠杆菌和大肠菌群的方法是在含有曲拉通7的乳糖TTC琼脂上进行膜过滤技术。该程序存在许多技术缺陷，以及在大肠菌群最新分类学方面的局限性，因此有必要评估替代方法。将两种替代检测方法，即显色培养基（m-ColiBlu24）和酶底物技术-DST检测（Colilert 18/定量盘）与ISO标准进行了比较，并关注分离株的表型特征。结果表明，ISO方法未能检测到相当比例的大肠菌群和大肠杆菌，而m-ColiBlu24和Colilert 18能在更短时间内得出结果，无需进一步确认步骤即可同时检测大肠杆菌和大肠菌群。
