Bonadonna Lucia, Cataldo Claudia, Chiaretti Gianluca, Coccia Annamaria, Semproni Maurizio
Ig Sanita Pubbl. 2005 Nov-Dec;61(6):569-84.
The ISO reference method, defined by the European Drinking Water Directive for the enumeration of total coliforms and Escherichia coli in water intended for human consumption, has various limitations, especially related to discrepancies observed with the new taxonomic classification of the coliform group. A study was therefore performed to compare the above reference method with another rapid method, the DST/Colilert, and to evaluate the phenotypical characteristics of isolated microrganisms. The ISO reference method failed to detect a significant proportion of coliforms and E. coli in water and furthermore, it enumerated microrganisms belonging to other groups. The DST/Colilert method was found instead to be a suitable alternative method for the detection of bacterial indicators.
欧洲饮用水指令定义的用于检测供人类饮用的水中总大肠菌群和大肠杆菌数量的ISO参考方法存在各种局限性，特别是与大肠菌群新分类法中观察到的差异有关。因此，开展了一项研究，将上述参考方法与另一种快速方法DST/Colilert进行比较，并评估分离出的微生物的表型特征。ISO参考方法未能检测出水中相当一部分的大肠菌群和大肠杆菌，此外，它还将属于其他菌群的微生物计算在内。相反，DST/Colilert方法被发现是检测细菌指标的一种合适的替代方法。
