Luczkiewicz M, Cisowski W, Kaiser P, Ochocka R, Piotrowski A
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdańsk, Gdańsk-Wrzeszcz, Poland.
Acta Pol Pharm. 2001 Sep-Oct;58(5):373-9.
Phenolic acids present in mistletoe plants collected from various hosts were analysed with the use of HPLC. The following numbers of compounds were found in the mistletoe plant material gathered from respective hosts: Sorbus aucuparia- 12 compounds; Acer plantanoides--14 compounds: Malus domestica, Pyrus communis and Populus nigra--13 compounds each; Quercus robur--15 compounds. Altogether 21 phenolic acids were chromatographically identified in the tested material. The compounds were either free or combined as esters or glycosides. Comparative chromatography revealed qualitative differences in the investigated compounds between the various plant materials. For example o-coumaric acid was only found in mistletoe hosted by Quercus robur. Digallic acid was only found in the plant material hosted by Acer plantanoides. Qualitative and quantitative composition of mistletoes hosted by Malus domestica and Pyrus communis showed considerable similarities as far as phenolic acids were concerned. Moreover. vanillic acid. absent in all other batches of plant material, seemed to be characteristic of the above mistletoes. Quantitative HPLC analysis demonstrated a considerable content of salicylic acid (39.55 mg%) in mistletoe hosted by Sorbus aucuparia. Apart from the above material, this compound was only present in small quantities in plants hosted by Populus nigra (15.63 mg%) and Quercus robur (2.63 mg%).
利用高效液相色谱法(HPLC)分析了从不同寄主采集的槲寄生植物中的酚酸。从各个寄主采集的槲寄生植物材料中发现的化合物数量如下:花楸——12种化合物;挪威槭——14种化合物;苹果、欧洲梨和黑杨——各13种化合物;夏栎——15种化合物。在测试材料中通过色谱法共鉴定出21种酚酸。这些化合物要么是游离的,要么以酯或糖苷的形式结合。对比色谱分析显示,不同植物材料中所研究的化合物存在质的差异。例如,邻香豆酸仅在夏栎寄主的槲寄生中发现。没食子酸仅在挪威槭寄主的植物材料中发现。就酚酸而言,苹果和欧洲梨寄主的槲寄生的定性和定量组成显示出相当大的相似性。此外,香草酸在所有其他批次的植物材料中均不存在,似乎是上述槲寄生的特征。高效液相色谱法定量分析表明,花楸寄主的槲寄生中水杨酸含量相当高(39.55毫克%)。除上述材料外,该化合物仅在黑杨寄主的植物(15.63毫克%)和夏栎寄主的植物(2.63毫克%)中少量存在。