Ekiert H, Szewczyk A, Kuś A
Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Faculty of Pharmacy, Kraków, Poland.
Pharmazie. 2009 Oct;64(10):694-6.
Eight phenolic acids were determined using HPLC method in methanolic extracts of Ruta graveolens L. (Rutaceae) shoots cultured in vitro on four variants of Linsmaier and Skoog (LS) medium differing in contents of growth regulators, NAA and BAP (ranging between 0.1-3.0 mg/l). Four compounds: protocatechuic, vanillic, syringic and p-coumaric acid were detected and quantified. The total content of metabolites was dependent on LS medium variants. The contents of protocatechuic acid, quantitatively dominating on all tested LS medium variants, were considerable (from 67.15 to 93.24 mg/100 g d.w.) in comparison with its contents in the plant material under analysis (46.36 to 218.27 mg/ 100 g d.w.). This is the first report of the isolation of protocatechuic acid from an in vitro plant culture.
采用高效液相色谱法测定了在四种生长调节剂NAA和BAP含量不同（范围为0.1 - 3.0 mg/l）的林氏和斯库格（LS）培养基变体上体外培养的芸香科植物芸香嫩枝甲醇提取物中的8种酚酸。检测并定量了4种化合物：原儿茶酸、香草酸、丁香酸和对香豆酸。代谢物的总含量取决于LS培养基变体。与被分析植物材料中的含量（46.36至218.27 mg/100 g干重）相比，原儿茶酸的含量在所有测试的LS培养基变体上在数量上占主导地位，含量相当可观（从67.15至93.24 mg/100 g干重）。这是首次从体外植物培养物中分离出原儿茶酸的报道。
