海参糖胺聚糖对刺激内皮细胞中组织因子和血栓调节蛋白表达的影响
[Effects of holothuria glycosaminoglycan on the expression of tissue factor and thrombomodulin in stimulated endothelial cells].
作者信息
Li Z, Wang X, Wang H, Guo W, Liu Y, Qu B, Guo X, Chu H
机构信息
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2000 Apr;21(4):201-4.
OBJECTIVE
To study the mechanism of antithrombotic effects of holothuria glycosaminoglycan (GAG).
METHODS
Endothelial cells from human umbilical vein which were pre-induced with 1 mg/L lipopolysaccharide, were treated by GAG (1 mg/L, 5 mg/L, and 10 mg/L, respectively) and 5 mg/L heparin as a control for 6 hours. Procoagulant activity (PCA), the expressions of tissue factor (TF) antigen and thrombomodulin (TM) antigen and their mRNA transcriptions were investigated.
RESULTS
GAG could down-regulate the expression of TF antigen and mRNA, up-regulate the expression of TM antigen and mRNA, down-regulate the PCA of stimulated endothelial cells.
CONCLUSION
Down-regulating TF expression and up-regulating TM expression of stimulated endothelial cells may be one of the mechanisms of GAG antithrombosis.
目的
研究海参糖胺聚糖（GAG）的抗血栓形成作用机制。
方法
用1mg/L脂多糖预诱导的人脐静脉内皮细胞，分别用GAG（1mg/L、5mg/L和10mg/L）及5mg/L肝素作为对照处理6小时。检测促凝血活性（PCA）、组织因子（TF）抗原和血栓调节蛋白（TM）抗原的表达及其mRNA转录情况。
结果
GAG可下调TF抗原和mRNA的表达，上调TM抗原和mRNA的表达，下调刺激后内皮细胞的PCA。
结论
下调刺激后内皮细胞的TF表达并上调TM表达可能是GAG抗血栓形成的机制之一。
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