日托中心儿童中抗蓝氏贾第鞭毛虫血清抗体的检测

Detection of anti-Giardia lamblia serum antibody among children of day care centers.

作者信息

Guimarães Semíramis, Sogayar Maria Ignês L

机构信息

Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brasil.

出版信息

Rev Saude Publica. 2002 Feb;36(1):63-8. doi: 10.1590/s0034-89102002000100010.

DOI:10.1590/s0034-89102002000100010

PMID:11887231

Abstract

OBJECTIVES

To detect anti-Giardia lamblia serum antibodies in healthy children attending public day care centers and to assess serological tests as tools for estimating the prevalence of G. lamblia in endemic areas.

METHODS

Three separate stool specimens and filter paper blood samples were collected from 147 children ranging from 0 to 6 years old. Each stool sample was processed using spontaneous sedimentation and zinc sulfate flotation methods. Blood samples were tested by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA) for Giardia IgG.

RESULTS AND CONCLUSIONS

Of 147 individuals tested, 93 (63.3%) showed Giardia cysts in their feces. Using IIF and ELISA, serum antibodies were detected in 93 (63.3%) and 100 (68%) samples, respectively. Sensitivity of IIF and ELISA was 82% and 72%, respectively. However, ELISA revealed to be less specific (39%) than IIF (70%). IIF also showed a higher concordance with microscopic examination than ELISA.

摘要

目的

检测在公立日托中心接受照料的健康儿童体内抗蓝氏贾第鞭毛虫血清抗体，并评估血清学检测作为估计流行地区蓝氏贾第鞭毛虫感染率工具的有效性。

方法

收集了147名年龄在0至6岁儿童的三份独立粪便样本和滤纸血样。每份粪便样本采用自然沉淀法和硫酸锌浮选法进行处理。血样通过间接免疫荧光法（IIF）和酶联免疫吸附测定法（ELISA）检测贾第鞭毛虫IgG。

结果与结论

在147名受测个体中，93人（63.3%）粪便中发现了贾第鞭毛虫囊肿。使用IIF和ELISA检测，分别在93份（63.3%）和100份（68%）样本中检测到血清抗体。IIF和ELISA的敏感性分别为82%和72%。然而，ELISA的特异性（39%）低于IIF（70%）。与ELISA相比，IIF与显微镜检查的一致性也更高。