Hassan M M, Farghaly A M, Darwish R A, Shoukrany N, el-Hayawan I A, Nassar A K
Department of Parasitology, Faculty of Medicine, Zagazig University, Egypt.
J Egypt Soc Parasitol. 1995 Apr;25(1):175-82.
A double antibody sandwich ELISA technique, using a chromatography purified Giardia antiserum, was applied to detect faecal antigen in patients infected with Giardia lamblia before and after treatment. The assay could detect antigens in 98% of infected cases with false positive reactions in 3 cases infected with E. histolytica. There was a significant direct relation between the antigen level in stool samples and the number of Giardia cysts. The mean level of copro-antigen was slightly lower in children, below 10 years, than in older patients, without significant difference. On the other hand, the lowest cyst count was noticed in elder patients, over 20 years. The level of faecal antigens decreased significantly after successful treatment in patients with giardiasis. It was concluded that detection of Giardia antigens by ELISA technique in the stool samples was a highly sensitive (98%) and specific (91%) diagnostic method. It is also considered as a good monitor for treatment success.
采用一种双抗体夹心酶联免疫吸附测定（ELISA）技术，使用经色谱纯化的贾第虫抗血清，对感染蓝氏贾第鞭毛虫的患者治疗前后的粪便抗原进行检测。该检测方法能够在98%的感染病例中检测到抗原，在3例感染溶组织内阿米巴的病例中出现假阳性反应。粪便样本中的抗原水平与贾第虫囊肿数量之间存在显著的直接关系。10岁以下儿童粪便抗原的平均水平略低于年龄较大的患者，但差异无统计学意义。另一方面，20岁以上的老年患者囊肿计数最低。贾第虫病患者成功治疗后，粪便抗原水平显著下降。结论是，通过ELISA技术检测粪便样本中的贾第虫抗原是一种高度敏感（98%）和特异（91%）的诊断方法。它也被认为是治疗成功的良好监测指标。
