[酶联免疫吸附测定法和自然沉降技术用于诊断秘鲁儿童粪便样本中的蓝氏贾第鞭毛虫感染]
[ELISA and spontaneous sedimentation technique for the diagnosis of Giardia lamblia infection in stool samples of Peruvian children].
作者信息
Rodríguez-Ulloa Claudia, Rivera-Jacinto Marco
机构信息
Departamento de Ciencias Biológicas, Universidad Nacional de Cajamarca.
出版信息
Salud Publica Mex. 2011 Nov-Dec;53(6):516-9.
OBJECTIVE
To compare a commercial coproantigen ELISA kit and the technique of spontaneous sedimentation in tube (TSET) for the diagnosis of Giardia lamblia in fecal specimens from children in a Peruvian endemic area.
MATERIAL AND METHODS
174 fecal samples were analyzed by TSET and 2nd Generation Giardia ELISA kit.
RESULTS
51 samples were positive by ELISA and 49 by TSET.
CONCLUSIONS
The ELISA was highly sensitive and specific, simple and fast. However, the very good agreement, high precision, low cost and ability to detect other intestinal parasites makes use of TSET recommended for laboratory diagnosis in endemic areas of Peru.
目的
比较一种商用粪抗原酶联免疫吸附测定（ELISA）试剂盒和试管内自然沉淀技术（TSET）对秘鲁流行地区儿童粪便标本中蓝氏贾第鞭毛虫的诊断效果。
材料与方法
采用TSET和第二代贾第虫ELISA试剂盒对174份粪便样本进行分析。
结果
ELISA检测阳性的样本有51份，TSET检测阳性的样本有49份。
结论
ELISA高度敏感且特异，操作简单快速。然而，TSET具有很好的一致性、高精度、低成本以及能够检测其他肠道寄生虫的能力，因此推荐在秘鲁流行地区用于实验室诊断。
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