Verpoorte Elisabeth
Sensors, Actuators & Microsystems Laboratory, Institute of Microtechnology, University of Neuchâtel, Neuchâtel, Switzerland.
Electrophoresis. 2002 Mar;23(5):677-712. doi: 10.1002/1522-2683(200203)23:5<677::AID-ELPS677>3.0.CO;2-8.
This review gives an overview of developments in the field of microchip analysis for clinical diagnostic and forensic applications. The approach chosen to review the literature is different from that in most microchip reviews to date, in that the information is presented in terms of analytes tested rather than microchip method. Analyte categories for which examples are presented include (i) drugs (quality control, seizures) and explosives residues, (ii) drugs and endogenous small molecules and ions in biofluids, (iii) proteins and peptides, and (iv) analysis of nucleic acids and oligonucleotides. Few cases of microchip analysis of physiological samples or other "real-world" matrices were found. However, many of the examples presented have potential application for these samples, especially with ongoing parallel developments involving integration of sample pretreatment onto chips and the use of fluid propulsion mechanisms other than electrokinetic pumping.
本综述概述了微芯片分析在临床诊断和法医应用领域的发展情况。与迄今为止大多数微芯片综述所采用的方法不同，本综述选择的文献呈现方式是根据所检测的分析物而非微芯片方法来提供信息。文中给出示例的分析物类别包括：（i）药物（质量控制、缉获量）和爆炸物残留；（ii）生物流体中的药物及内源性小分子和离子；（iii）蛋白质和肽；以及（iv）核酸和寡核苷酸分析。很少发现对生理样本或其他“实际”基质进行微芯片分析的案例。然而，文中给出的许多示例对这些样本具有潜在应用价值，特别是随着当前在芯片上集成样品预处理以及使用除电动泵以外的流体推进机制等并行发展。
