Boros László G, Cascante Marta, Lee Wai Nang Paul
Harbor-UCLA Research and Education Institute, UCLA School of Medicine, 1124 West Carson Street RB1, 90502, Torrance, CA 90502, USA.
Drug Discov Today. 2002 Mar 15;7(6):364-72. doi: 10.1016/s1359-6446(02)02179-7.
Metabolic profiling using stable-isotope tracer technology enables the measurement of substrate redistribution within major metabolic pathways in living cells. This technique has demonstrated that transformed human cells exhibit profound metabolic shifts and that some anti-cancer drugs produce their effects by forcing the reversion of these metabolic changes. By revealing tumor-specific metabolic shifts in tumor cells, metabolic profiling enables drug developers to identify the metabolic steps that control cell proliferation, thus aiding the identification of new anti-cancer targets and screening of lead compounds for anti-proliferative metabolic effects.
使用稳定同位素示踪技术进行代谢谱分析能够测量活细胞内主要代谢途径中的底物再分布。该技术已证明，转化的人类细胞表现出深刻的代谢变化，并且一些抗癌药物通过促使这些代谢变化逆转来发挥作用。通过揭示肿瘤细胞中肿瘤特异性的代谢变化，代谢谱分析使药物研发人员能够识别控制细胞增殖的代谢步骤，从而有助于确定新的抗癌靶点并筛选具有抗增殖代谢作用的先导化合物。
