Putzer H, Werenskiold K, Verfuerth C, Schreckenbach T
Max-Planck-Institut für Biochemie, Martinsried/München, FRG.
EMBO J. 1983;2(2):261-7. doi: 10.1002/j.1460-2075.1983.tb01415.x.
The influence of blue light on protein synthesis in spherulating Physarum polycephalum microplasmodia was studied using two-dimensional protein separation techniques. The starvation-induced plasmodium-spherule transition proceeds in the dark and is accompanied by the synthesis of 20 major differentiation-specific proteins as revealed by in vivo labelling with [35S]methionine. Three of these proteins are identical with cell wall components with respect to their mol. wts. (35 K, 34 K and 14 K) and isoelectric points. Spherulation is also accompanied by the appearance of 26 prominent differentiation-specific mRNA species translatable in the rabbit reticulocyte cell-free system. Six of the proteins synthesized in vitro co-migrate on two-dimensional gels with proteins labelled in vivo, two of them being cell wall components. Blue light, which inhibits spherulation completely, inhibits also the synthesis of spherule proteins and of spherule-specific mRNA activity. Only three protein components are induced by blue light, indicating that illumination does not induce a novel differentiated plasmodial state.
利用二维蛋白质分离技术研究了蓝光对多头绒泡菌微原质团形成孢子过程中蛋白质合成的影响。饥饿诱导的原质团-孢子转变在黑暗中进行，如用[35S]甲硫氨酸进行体内标记所示，该过程伴随着20种主要的分化特异性蛋白质的合成。其中三种蛋白质在分子量（35K、34K和14K）和等电点方面与细胞壁成分相同。孢子形成还伴随着26种突出的分化特异性mRNA种类的出现，这些mRNA可在兔网织红细胞无细胞系统中翻译。在体外合成的六种蛋白质在二维凝胶上与体内标记的蛋白质共迁移，其中两种是细胞壁成分。完全抑制孢子形成的蓝光也抑制孢子蛋白质的合成和孢子特异性mRNA活性。蓝光仅诱导三种蛋白质成分，表明光照不会诱导新的分化原质团状态。
