Schreckenbach T, Walckhoff B, Verfuerth C
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1009-13. doi: 10.1073/pnas.78.2.1009.
Blue light induces sporulation of Physarum polycephalum macroplasmodia and reversibly inhibits spherulation (sclerotization) of microplasmodia. Illuminated microplasmodia have an abnormal appearance. The photobiological responses of the plasmodia appear to be unaffected by the absence of yellow pigment in the white mutant strain used. Illumination of microplasmodial suspensions with blue light (lambda max approximately 465 nm) results also in an early effect on glucose metabolism: glucose consumption is reversibly inhibited. By using radioactive glucose it was shown that the main products formed are a water-insoluble glucan and the disaccharide trehalose. Inhibition of glucose consumption in the light results in decreased production of these two compounds. Illumination of microplasmodial suspensions also causes a reversible effect on the pH of the medium which is interpreted as a decreased production of a yet unidentified acid from glucose. The action spectrum of the light-induced pH response shows maxima near 390, 465, and 485 nm. It resembles the absorption spectrum of a flavoprotein and confirms the existence of a blue-light receptor in P. polycephalum microplasmodia.
蓝光可诱导多头绒泡菌大原质团形成孢子，并可逆性抑制小原质团的球形化（硬化）。受光照的小原质团外观异常。在所使用的白色突变菌株中，即使缺乏黄色色素，原质团的光生物学反应似乎也未受影响。用蓝光（最大波长约465nm）照射小原质团悬浮液，对葡萄糖代谢也会产生早期影响：葡萄糖消耗受到可逆性抑制。通过使用放射性葡萄糖表明，形成的主要产物是一种水不溶性葡聚糖和二糖海藻糖。光照下葡萄糖消耗的抑制导致这两种化合物的产量降低。照射小原质团悬浮液还会对培养基的pH产生可逆影响，这被解释为葡萄糖产生的一种尚未鉴定的酸减少。光诱导pH反应的作用光谱在390、465和485nm附近出现最大值。它类似于黄素蛋白的吸收光谱，并证实了多头绒泡菌小原质团中存在蓝光受体。
