博德特氏菌噬菌体中逆转录酶介导的嗜性转换

Reverse transcriptase-mediated tropism switching in Bordetella bacteriophage.

作者信息

Liu Minghsun, Deora Rajendar, Doulatov Sergei R, Gingery Mari, Eiserling Frederick A, Preston Andrew, Maskell Duncan J, Simons Robert W, Cotter Peggy A, Parkhill Julian, Miller Jeff F

机构信息

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.

出版信息

Science. 2002 Mar 15;295(5562):2091-4. doi: 10.1126/science.1067467.

DOI:10.1126/science.1067467

PMID:11896279

Abstract

Host-pathogen interactions are often driven by mechanisms that promote genetic variability. We have identified a group of temperate bacteriophages that generate diversity in a gene, designated mtd (major tropism determinant), which specifies tropism for receptor molecules on host Bordetella species. Tropism switching is the result of a template-dependent, reverse transcriptase-mediated process that introduces nucleotide substitutions at defined locations within mtd. This cassette-based mechanism is capable of providing a vast repertoire of potential ligand-receptor interactions.

摘要

宿主-病原体相互作用通常由促进基因变异的机制驱动。我们鉴定出了一组温和噬菌体，它们在一个名为mtd（主要嗜性决定因素）的基因中产生多样性，该基因决定了对宿主博德特氏菌属物种上受体分子的嗜性。嗜性转换是一个依赖模板、逆转录酶介导的过程的结果，该过程在mtd内的特定位置引入核苷酸替换。这种基于盒式结构的机制能够提供大量潜在的配体-受体相互作用。