Anderson Joseph, Reddy Vijaya B, Green Linda, Bitterman Pincas, Borok Raphael, Maggi-Galluzzi Christina, Montironi Rodolfo, Wick Mark, Gould Victor E, Gattuso Paolo
Department of Pathology, Rush-Presbyterian-St. Luke's Medical Center, Rush Medical College, 1653 West Congress Parkway, Jelke 582, Chicago, IL 60612, USA.
Breast J. 2002 Mar-Apr;8(2):101-7. doi: 10.1046/j.1524-4741.2002.08206.x.
Tumor expression of the proliferation marker (MIB-1) and the cell cycle-related protein (p27) may predict the biologic behavior of various human tumors. The purpose of this study was to evaluate the role of p27 and MIB-1 expression in predicting lymph node metastasis in male breast carcinomas (MBCs). We studied 67 patients with invasive MBC who had undergone modified radical mastectomy. Pathologic lymph node status was correlated with the p27 protein and the MIB-1 proliferation index. These factors were studied immunohistologically by standard methods. Men in this study ranged from 36 to 92 years of age (mean, 63 years); 43 (64%) were T1 lesions, and 24 (36%) were T2 lesions. Twenty-nine patients (43%) had positive nodes. p27 was expressed in 43 tumors (64%) and MIB-1 in 13 tumors (19.4%). Tumors with positive p27 showed positive lymph nodes in 10 cases (23%). In contrast, p27-negative tumors had positive lymph nodes in 18 cases (75%). Tumors positive for MIB-1 show positive lymph nodes in 11 cases (85%). However, when MIB-1 was negative, only 16 patients (30%) had positive lymph nodes. Multivariate logistic regression analysis confirmed the utility of MIB-1 overexpression in predicting lymph node metastasis ( p < 0.0006). Also, decreased p27 protein expression strongly correlates with lymph node metastasis ( p < or = 0.0001). Furthermore, when p27 was negative and MIB-1 was positive, 100% of the patients had positive lymph nodes. In contrast, when p27 was positive and MIB-1 was negative, only 12% of patients had positive lymph nodes. The reduced expression of the p27 protein and the overexpression of the MIB-1 proliferation index in this study show a significant correlation in predicting lymph nodes metastasis in MBCs.
增殖标志物（MIB-1）和细胞周期相关蛋白（p27）的肿瘤表达可能预测各种人类肿瘤的生物学行为。本研究的目的是评估p27和MIB-1表达在预测男性乳腺癌（MBC）淋巴结转移中的作用。我们研究了67例接受改良根治性乳房切除术的浸润性MBC患者。病理淋巴结状态与p27蛋白和MIB-1增殖指数相关。通过标准方法对这些因素进行免疫组织学研究。本研究中的男性年龄在36至92岁之间（平均63岁）；43例（64%）为T1期病变，24例（36%）为T2期病变。29例患者（43%）有阳性淋巴结。43例肿瘤（64%）表达p27，13例肿瘤（19.4%）表达MIB-1。p27阳性的肿瘤中有10例（23%）出现阳性淋巴结。相比之下，p27阴性的肿瘤中有18例（75%）出现阳性淋巴结。MIB-1阳性的肿瘤中有11例（85%）出现阳性淋巴结。然而，当MIB-1为阴性时，只有16例患者（30%）有阳性淋巴结。多因素逻辑回归分析证实MIB-1过表达在预测淋巴结转移方面的效用（p<0.0006）。此外，p27蛋白表达降低与淋巴结转移密切相关（p≤0.0001）。此外，当p27为阴性且MIB-1为阳性时，100%的患者有阳性淋巴结。相比之下，当p27为阳性且MIB-1为阴性时，只有12%的患者有阳性淋巴结。本研究中p27蛋白表达降低和MIB-1增殖指数过表达在预测MBC淋巴结转移方面显示出显著相关性。
