来自黏金黄杆菌的安布勒A类超广谱β-内酰胺酶CGA-1的分子和生化特征
Molecular and biochemical characterization of Ambler class A extended-spectrum beta-lactamase CGA-1 from Chryseobacterium gleum.
作者信息
Bellais Samuel, Naas Thierry, Nordmann Patrice
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France.
出版信息
Antimicrob Agents Chemother. 2002 Apr;46(4):966-70. doi: 10.1128/AAC.46.4.966-970.2002.
Abstract
Antibiotic susceptibility testing by disk diffusion of a Chryseobacterium gleum isolate, strain CIP 103039, showed a typical synergy image between clavulanic acid and expanded-spectrum cephalosporins. Shotgun cloning gave a recombinant plasmid in Escherichia coli that produced a beta-lactamase, CGA-1, with a pI value of 8.9 that conferred resistance to most penicillins (except ureidopenicillins) and narrow-spectrum cephalosporins and an intermediate susceptibility to expanded-spectrum cephalosporins and aztreonam. The CGA-1 amino acid sequence shared only 60% amino acid identity with CME-1 and CME-2 from Chryseobacterium meningosepticum, the most closely related beta-lactamases. CGA-1 was very likely chromosome encoded. It is a novel member of the PER subgroup of Ambler class A beta-lactamases (Bush functional group 2be).
摘要
通过纸片扩散法对一株戈氏金黄杆菌（Chryseobacterium gleum）分离株（菌株CIP 103039）进行抗生素敏感性测试，结果显示克拉维酸与广谱头孢菌素之间呈现典型的协同作用图像。鸟枪法克隆在大肠杆菌中获得了一个重组质粒，该质粒产生一种β-内酰胺酶CGA-1，其pI值为8.9，对大多数青霉素（除脲基青霉素外）和窄谱头孢菌素具有抗性，对广谱头孢菌素和氨曲南具有中等敏感性。CGA-1的氨基酸序列与来自脑膜败血金黄杆菌（Chryseobacterium meningosepticum）的最密切相关的β-内酰胺酶CME-1和CME-2仅具有60%的氨基酸同一性。CGA-1很可能是染色体编码的。它是安布勒A类β-内酰胺酶PER亚组（布什功能组2be）的一个新成员。
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本文引用的文献
1
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Antimicrob Agents Chemother. 2001 Dec;45(12):3595-8. doi: 10.1128/AAC.45.12.3595-3598.2001.
2
Genetic diversity of carbapenem-hydrolyzing metallo-beta-lactamases from Chryseobacterium (Flavobacterium) indologenes.产吲哚金黄杆菌（黄杆菌属）中碳青霉烯水解金属β-内酰胺酶的遗传多样性
Antimicrob Agents Chemother. 2000 Nov;44(11):3028-34. doi: 10.1128/AAC.44.11.3028-3034.2000.
3
The high resolution crystal structure for class A beta-lactamase PER-1 reveals the bases for its increase in breadth of activity.
J Biol Chem. 2000 Sep 8;275(36):28075-82. doi: 10.1074/jbc.M003802200.
4
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5
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6
The taxonomy, ecology and cultivation of bacterial genera belonging to the family Flavobacteriaceae.
Int J Food Microbiol. 1999 Dec 15;53(2-3):81-94. doi: 10.1016/s0168-1605(99)00162-2.
7
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Antimicrob Agents Chemother. 2000 Jan;44(1):1-9. doi: 10.1128/AAC.44.1.1-9.2000.
8
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Antimicrob Agents Chemother. 1999 Sep;43(9):2193-9. doi: 10.1128/AAC.43.9.2193.
9
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Antimicrob Agents Chemother. 1999 Apr;43(4):769-76. doi: 10.1128/AAC.43.4.769.
10
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Biochem J. 1998 Mar 15;330 ( Pt 3)(Pt 3):1443-9. doi: 10.1042/bj3301443.
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