Tisdall P A, Roberts G D, Anhalt J P
J Clin Microbiol. 1979 Oct;10(4):506-14. doi: 10.1128/jcm.10.4.506-514.1979.
Identification of 18 mycobacterial species was performed by analysis of profiles obtained by using gas-liquid chromatography. Organisms were saponified in methanolic NaOH, and the reaction mixture was treated with BF(3) in methanol and extracted with a hexane-chloroform mixture. An identification scheme was developed from 128 stock strains and tested against a collection of 79 clinical isolates. By using gas-liquid chromatographic profiles alone, 58% of specimens were correctly identified to species level, and an additional 41% were correctly identified to a group of two or three organisms. Use in a clinical laboratory over a 2-month period proved chromatography to be as accurate as and more rapid than concurrent biochemical testing. Of 81 isolates tested, 64% were identified to species level by chromatography alone. An additional 35% were differentiated to the same groups of two or three organisms as found in our analysis of stock strains. These groups consisted of: Mycobacterium tuberculosis, M. bovis, and M. xenopi; M. avium complex, M. gastri, and M. scrofulaceum; or M. fortuitum and M. chelonei. Identification to species level from these groups could usually be done by colonial morphology alone and could always be done by the addition of one selected biochemical test. This study demonstrated the practical application of gas-liquid chromatography in the identification of mycobacteria in a clinical laboratory. In particular, all strains of M. gordonae and M. kansasii were identified to species level. M. tuberculosis was definitively identified in 85% of cases. When it could not be definitely identified, the only alternatives were M. bovis and M. xenopi, both of which are rare causes of infection.
通过分析气液色谱法获得的图谱对18种分枝杆菌进行鉴定。将菌株在甲醇氢氧化钠中皂化，反应混合物用甲醇中的BF(3)处理，并用己烷 - 氯仿混合物萃取。从128株标准菌株建立了一种鉴定方案，并对79株临床分离株进行了测试。仅使用气液色谱图谱，58%的标本被正确鉴定到种水平，另外41%被正确鉴定到由两到三种菌组成的组。在临床实验室使用两个月的结果证明，色谱法与同时进行的生化检测一样准确且速度更快。在测试的81株分离株中，仅通过色谱法64%被鉴定到种水平。另外35%被区分到与我们对标准菌株分析中发现的相同的由两到三种菌组成的组。这些组包括：结核分枝杆菌、牛分枝杆菌和偶发分枝杆菌；鸟分枝杆菌复合群、胃分枝杆菌和瘰疬分枝杆菌；或偶然分枝杆菌和龟分枝杆菌。从这些组中鉴定到种水平通常仅通过菌落形态即可完成，并且总是可以通过添加一项选定的生化试验来完成。本研究证明了气液色谱法在临床实验室分枝杆菌鉴定中的实际应用。特别是，所有戈登分枝杆菌和堪萨斯分枝杆菌菌株都被鉴定到种水平。85%的病例中结核分枝杆菌得到明确鉴定。当无法明确鉴定时，唯一的可能性是牛分枝杆菌和偶发分枝杆菌，这两种都是罕见的感染原因。
