Ohashi D K, Wade T J, Mandle R J
J Clin Microbiol. 1977 Nov;6(5):469-73. doi: 10.1128/jcm.6.5.469-473.1977.
The methanolic tetramethylammonium hydroxide whole-cell lysates of nine species of mycobacteria and the "rhodochrous complex" were examined by gas-liquid chromatography. The gas chromatographic patterns produced 10 characteristic chromatographic groups that corresponded to the 10 species studied. The gas chromatograms of Mycobacterium tuberculosis were very easily distinguished from the other mycobacterial strains by high levels of a component that eluted much later than the other components. The remaining nine species could be distinguished on the basis of characteristic components and by different amounts of components common to more than one species. This study demonstrated that direct gas-liquid chromatographic characterization of M. tuberculosis and other myobacterial species was not only feasible but practical in the clinical laboratory.
采用气液色谱法对9种分枝杆菌和“红球菌复合体”的甲醇氢氧化四甲铵全细胞裂解物进行了检测。气相色谱图谱产生了10个特征色谱组，与所研究的10个物种相对应。结核分枝杆菌的气相色谱图很容易与其他分枝杆菌菌株区分开来，因为有一种成分的含量很高，其洗脱时间比其他成分晚得多。其余9个物种可以根据特征成分以及一种以上物种共有的成分的不同含量来区分。这项研究表明，结核分枝杆菌和其他分枝杆菌物种的直接气液色谱特征分析在临床实验室中不仅可行而且实用。
