分枝杆菌的气相色谱表征：脂肪酸和三氟乙酰化全细胞甲醇解产物的分析
Gas chromatographic characterization of mycobacteria: analysis of fatty acids and trifluoroacetylated whole-cell methanolysates.
作者信息
Larsson L, Mårdh P A
出版信息
J Clin Microbiol. 1976 Feb;3(2):81-5. doi: 10.1128/jcm.3.2.81-85.1976.
Abstract
Mycobacterium avium, M. bovis strain Bacillus Calmette-Guerin (BCG), M. kansasii, and M. tuberculosis were studied by gas-liquid chromatography. Methylated fatty acids of the mycobacterial lipids and trifluoroacetyl (TFA) derivatives of whole-cell methanolysates were analyzed. Both the fatty acid and the TFA chromatograms showed reproducible differences between the various mycobacteria studied. Chromatograms from different strains of one and the same species showed negligible differences. Fatty acid methyl esters and TFA methyl glycosides are probably the main constituents of the TFA chromatograms. TFA derivatives are easily prepared and the method provides a potential tool for species indentification of mycobacteria.
摘要
采用气液色谱法对鸟分枝杆菌、卡介苗（牛分枝杆菌菌株）、堪萨斯分枝杆菌和结核分枝杆菌进行了研究。分析了分枝杆菌脂质的甲基化脂肪酸和全细胞甲醇解产物的三氟乙酰（TFA）衍生物。脂肪酸色谱图和TFA色谱图均显示，在所研究的各种分枝杆菌之间存在可重复的差异。同一菌种不同菌株的色谱图显示差异可忽略不计。脂肪酸甲酯和TFA甲基糖苷可能是TFA色谱图的主要成分。TFA衍生物易于制备，该方法为分枝杆菌的菌种鉴定提供了一种潜在工具。
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