Robin V, Irurzun A, Amoros M, Boustie J, Carrasco L
UPRES, Pharmacochimie de Molécules de Synthèse et de Substances Naturelles, Faculté de Pharmacie, Rennes, France.
Antivir Chem Chemother. 2001 Sep;12(5):283-91. doi: 10.1177/095632020101200503.
The search for antiviral agents against vesicular stomatitis virus, herpes simplex virus type 1 and poliovirus type 2 in plants extracts, led to the isolation of two antipoliovirus flavonoids from the medicinal plant Psiadia dentata (Cass.) DC, Asteraceae: 3-methylkaempferol and 3,4'-dimethylkaempferol. The antipoliovirus activity of both compounds was estimated by comparison with 3-methylquercetin, guanidine and Ro-090179. The most potent inhibitor of poliovirus replication was 3-methylkaempferol, and therefore we investigated its mechanism of action. We showed, using the inhibition of [3H]uridine incorporation in viral RNA and performing a dot-blot with one RNA probe specific for the poliovirus genomic strand RNA, that 3-methylkaempferol inhibits the genomic RNA synthesis of poliovirus.
在植物提取物中寻找抗水疱性口炎病毒、1型单纯疱疹病毒和2型脊髓灰质炎病毒的抗病毒剂，导致从菊科药用植物齿叶翼茎草（Psiadia dentata (Cass.) DC）中分离出两种抗脊髓灰质炎病毒黄酮类化合物：3-甲基山奈酚和3,4'-二甲基山奈酚。通过与3-甲基槲皮素、胍和Ro-090179比较，评估了这两种化合物的抗脊髓灰质炎病毒活性。脊髓灰质炎病毒复制的最有效抑制剂是3-甲基山奈酚，因此我们研究了其作用机制。我们通过抑制病毒RNA中[3H]尿苷的掺入，并使用一种针对脊髓灰质炎病毒基因组链RNA的RNA探针进行斑点印迹，表明3-甲基山奈酚抑制脊髓灰质炎病毒的基因组RNA合成。
