Albrecht Benno, Failla Antonio Virgilio, Schweitzer Andreas, Cremer Christoph
Applied Optics and Information Processing, Kirchhoff Institute for Physics, University of Heidelberg, Germany.
Appl Opt. 2002 Jan 1;41(1):80-7. doi: 10.1364/ao.41.000080.
For an improved understanding of the structural basis of cellular mechanisms, it is highly desirable to develop methods for a detailed topological analysis of biological nanostructures and their dynamics in the interior of three-dimensionally conserved cells. We present a method of far-field laser fluorescence microscopy to measure relative axial positions of pointlike fluorescent targets and the distance between each target in the range of a few nanometers. The physical principle behind this approach can be extended to the determination of three-dimensional (3D) positions and 3D distances between any number of objects that can be discriminated owing to their spectral signature, thus allowing topological measurements so far regarded to be beyond the capabilities of light microscopy.
为了更好地理解细胞机制的结构基础，非常需要开发一些方法，以便对生物纳米结构及其在三维保守细胞内部的动力学进行详细的拓扑分析。我们提出了一种远场激光荧光显微镜方法，用于测量点状荧光目标的相对轴向位置以及每个目标之间在几纳米范围内的距离。这种方法背后的物理原理可以扩展到确定任意数量物体之间的三维（3D）位置和三维距离，这些物体由于其光谱特征而能够被区分，从而实现迄今为止被认为超出光学显微镜能力范围的拓扑测量。
