Sakai Masatoshi, Tsuda Hiroshi, Tanebe Kyoko, Sasaki Yasushi, Saito Shigeru
Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Sugitani Toyama-shi, Toyama, Japan.
Am J Reprod Immunol. 2002 Feb;47(2):91-7. doi: 10.1034/j.1600-0897.2002.1o020.x.
It has been reported that T-helper (Th) 2 dominance in normal pregnancy shifts to Th1 dominance in preeclampsia. Peripheral blood mononuclear cell (PBMC) production of interleukin (IL)-12, which induce Th1 responses, has not been compared between these clinical states.
Peripheral blood mononuclear cell from 35 non-pregnant women, 35 healthy pregnant women, 12 mildly preeclamptic patients, and 15 severely preeclamptic patients were cultured for 24 hr. IL-12 secretion was determined by enzyme-linked immunosorbent assay (ELISA). Th1/Th2 ratios in PBMC were determined flow-cytometrically, and the amounts of HLA-DR and CD14 expression on the monocytes were obtained by flow cytometry.
Peripheral blood mononuclear cell from healthy pregnant subjects secreted less IL-12 than non-pregnant women. PBMC from severely preeclamptic patients secreted more IL-12 than those from healthy pregnant subjects, while IL-12 secretion in mild preeclampsia resembled secretion in normal pregnancy. Th1/Th2 ratios correlated were positively with IL-12. Increased HLA-DR antigens and reduced CD14 expression, suggesting monocyte activation, were observed in preeclamptic patients, although monocyte counts were unchanged.
Decreased IL-12 secretion by PBMC may cause Th2 dominance in normal pregnancy, while increased IL-12 secretion by activated monocytes may cause Th1 dominance in preeclampsia.
据报道，正常妊娠时辅助性T细胞（Th）2占优势，而子痫前期则转变为Th1占优势。尚未对这两种临床状态下诱导Th1反应的白细胞介素（IL）-12在外周血单个核细胞（PBMC）中的产生情况进行比较。
将35名未孕女性、35名健康孕妇、12名单纯轻度子痫前期患者和15名重度子痫前期患者的外周血单个核细胞培养24小时。采用酶联免疫吸附测定（ELISA）法测定IL-12分泌水平。通过流式细胞术测定PBMC中的Th1/Th2比值，并通过流式细胞仪检测单核细胞上HLA-DR和CD14的表达量。
健康孕妇的外周血单个核细胞分泌的IL-12少于未孕女性。重度子痫前期患者的PBMC分泌的IL-12多于健康孕妇，而轻度子痫前期患者的IL-12分泌情况与正常妊娠相似。Th1/Th2比值与IL-12呈正相关。子痫前期患者虽然单核细胞计数未变，但可见HLA-DR抗原增加和CD14表达减少，提示单核细胞活化。
PBMC分泌IL-12减少可能导致正常妊娠时Th2占优势，而活化单核细胞分泌IL-12增加可能导致子痫前期时Th1占优势。
