Lord G A, Lim C K, Warnakulasuriya S, Peters T J
MRC Bioanalytical Science Group, School of Biological and Chemical Sciences, Birkbeck, University of London, London, UK.
Addict Biol. 2002 Jan;7(1):99-102. doi: 10.1080/13556210120091455.
A capillary zone electrophoretic (CZE) method for the rapid analysis of the major alkaloids (arecoline and guavacoline) in areca nut extract is described. Areca nuts were pulverized and then extracted with water by sonication in a water bath. After centrifugation, the supernatant was analysed on a fused-silica capillary with 100 mM ammonium acetate-acetic acid (pH 4.6) as the running buffer at a voltage of 20 kV and temperature of 30 degrees C. The method is applicable to the analysis of alkaloids in the nut, commercial preparations (pan masala) and in the saliva of areca nut chewers.
描述了一种用于快速分析槟榔提取物中主要生物碱（槟榔碱和去甲槟榔碱）的毛细管区带电泳（CZE）方法。将槟榔研磨成粉，然后在水浴中通过超声用水提取。离心后，取上清液在熔融石英毛细管上进行分析，运行缓冲液为100 mM醋酸铵-醋酸（pH 4.6），电压为20 kV，温度为30℃。该方法适用于分析槟榔果、商业制剂（潘马斯ala）以及嚼槟榔者唾液中的生物碱。
