基于芯片的蛋白质组学：最新的芯片挑战。

Array-based proteomics: the latest chip challenge.

作者信息

Albala J S

机构信息

Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.

出版信息

Expert Rev Mol Diagn. 2001 Jul;1(2):145-52. doi: 10.1586/14737159.1.2.145.

DOI:10.1586/14737159.1.2.145

PMID:11901809

Abstract

Array-based protein technologies are emerging for basic biological research, molecular diagnostics and therapeutic development with the potential of providing parallel functional analysis of hundreds or perhaps hundreds of thousands of proteins simultaneously. Array-based methods are becoming prevalent within proteomics research due to the desire to analyze proteins in an analogous format to that of the DNA microarray. Novel protein biochips are under development in academic laboratories and emerging biotechnology companies to advance the pace and scope of scientific discovery. This review will define array-based proteomics, its current applications and future directions, as well as examine the challenges and limitations of this projected billion dollar industry.

摘要

基于阵列的蛋白质技术正在兴起，用于基础生物学研究、分子诊断和治疗开发，有潜力同时对成百甚至可能成千上万种蛋白质进行平行功能分析。由于希望以类似于DNA微阵列的形式分析蛋白质，基于阵列的方法在蛋白质组学研究中越来越普遍。学术实验室和新兴生物技术公司正在开发新型蛋白质生物芯片，以加快科学发现的步伐和扩大其范围。本综述将定义基于阵列的蛋白质组学、其当前应用和未来方向，并探讨这个预计价值数十亿美元行业所面临的挑战和局限性。