Conway D P, Mathis G F, Lang M
Conway Associates, Asbury, New Jersey 08802, USA.
Poult Sci. 2002 Mar;81(3):353-5. doi: 10.1093/ps/81.3.353.
The effect of diclazuril medication on the development of natural immunity to Eimeria tenella was determined. Birds in two treatments, nonexposed, non-challenged (NENC) and nonexposed, challenged (NEC), were from a holding group raised under conditions designed to prevent coccidial infection. Birds in five other treatments, from the same hatch and source as birds in the first two treatments, were from a floor pen study involving a natural exposure to Eimeria spp. These birds were assigned to the current study based on their respective treatments in the original floor pen study as follows: unmedicated (UNM), 66 ppm salinomycin (SAL) + 50 ppm roxarsone in the starter and grower diets to Day 28 (SAL 28), 66 ppm SAL + 50 ppm roxarsone in the starter diet and 1 ppm diclazuril (DIC) in the grower diet to Day 28 (DIC 28) or to Day 35 (DIC 35), or in the grower and finisher diets to Day 42 (DIC 42). Each treatment comprised three floor pens of 10 birds (female) in a randomized complete block design. All birds were fed an UNM finisher diet during the 7-d challenge study. Birds in each treatment, except NENC, were individually inoculated (p.o.) with 1 x 10(5) Eimeria tenella sporulated oocysts on Day 1. Based on bird performance and cecal lesion scores, birds in the DIC 35 and 42 treatments had a low immunity to the challenge infection, birds in the DIC 28 treatment were partially immunized, and the highest levels of immunity were observed in the UNM and SAL 28 treatments.
测定了地克珠利药物对鸡对柔嫩艾美耳球虫自然免疫力发展的影响。两种处理组的鸡，即未接触、未攻毒组（NENC）和未接触、攻毒组（NEC），来自于在旨在预防球虫感染的条件下饲养的育雏组。另外五种处理组的鸡，与前两组鸡来自同一批次和来源，来自于一项涉及自然接触艾美耳属球虫的地面平养研究。这些鸡根据它们在原始地面平养研究中的各自处理情况被分配到当前研究中，如下：未用药组（UNM）、在雏鸡和生长鸡日粮中添加66 ppm盐霉素（SAL）+50 ppm洛克沙胂至第28天（SAL 28）、在雏鸡日粮中添加66 ppm SAL +50 ppm洛克沙胂且在生长鸡日粮中添加1 ppm地克珠利至第28天（DIC 28）或至第35天（DIC 35）、或在生长鸡和育肥鸡日粮中添加至第42天（DIC 42）。每种处理包括三个地面平养栏，每个栏中有10只（雌性）鸡，采用随机完全区组设计。在为期7天的攻毒研究期间，所有鸡均饲喂未用药的育肥鸡日粮。除NENC组外，每种处理组中的鸡在第1天均经口单独接种1×10⁵个柔嫩艾美耳球虫孢子化卵囊。根据鸡的生产性能和盲肠病变评分，DIC 35和42处理组的鸡对攻毒感染的免疫力较低，DIC 28处理组的鸡部分免疫，而在UNM和SAL 28处理组中观察到最高水平的免疫力。
