超临界流体萃取用于血浆中特定抗炎药物分析的条件研究。
A study of the conditions of the supercritical fluid extraction in the analysis of selected anti-inflammatory drugs in plasma.
作者信息
Klime Jiri, Sochor Jaroslav, Kríz Josef
机构信息
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic.
出版信息
Farmaco. 2002 Feb;57(2):117-22. doi: 10.1016/s0014-827x(01)01182-x.
Supercritical fluid extraction (SFE) was employed to analyze selected anti-inflammatory drugs in plasma. Evaluation of selected drugs (ibuprofen, indomethacin, and flufenamic acid) was performed using the HPLC method on columns with the reverse phase C-18 and detection in the UV region of the spectrum. A study of the conditions of SFE carried out for 30 min at 50 degrees C investigated the magnitude of the pressure of carbon dioxide suitable for drug extraction, the selection of the collecting solvent, and the modification of CO2 with an organic solvent. The results of the study made it possible to determine the optimal procedure for SFE of ibuprofen, indomethacin, and flufenamic acid from plasma, which renders their HPLC quantification possible.
采用超临界流体萃取（SFE）法分析血浆中选定的抗炎药物。使用高效液相色谱法在反相C-18柱上对选定药物（布洛芬、吲哚美辛和氟芬那酸）进行评估，并在光谱的紫外区域进行检测。在50℃下进行30分钟的超临界流体萃取条件研究，考察了适合药物萃取的二氧化碳压力大小、收集溶剂的选择以及用有机溶剂对二氧化碳进行改性。该研究结果使得确定从血浆中萃取布洛芬、吲哚美辛和氟芬那酸的超临界流体萃取最佳程序成为可能，从而实现对它们的高效液相色谱定量分析。
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