Bettinger Ina, Thanos Solon, Paulus Werner
Institute of Neuropathology, University Hospital Münster, Domagkstr. 19, 48129 Münster, Germany.
Acta Neuropathol. 2002 Apr;103(4):351-5. doi: 10.1007/s00401-001-0472-x. Epub 2001 Dec 8.
Diffuse astrocytic gliomas extensively infiltrate brain tissue and contain numerous microglial cells, but it is unknown whether these two characteristic features are pathogenetically related. We therefore studied the effects of murine microglial cells on motility of GL261 mouse glioma cells using Boyden chamber assays. In the presence of microglia, glioma cell migration occurred earlier, and after 48 h it was threefold higher as compared to incubations without microglia. This effect was mediated by substances released from microglia, because similar effects were observed by microglia-conditioned medium, and it was specific to microglia, because oligodendroglia and endothelial cells only weakly stimulated glioma cell migration. Microglia activating substances (GM-CSF, LPS) led to a further increase of motility. These data support the notion that microglia accumulation in diffuse glial tumors does not merely represent a nonspecific reaction to tissue injury, but reflects participation of these cells in supporting and promoting the invasive phenotype of astrocytoma cells.
弥漫性星形胶质细胞瘤广泛浸润脑组织并含有大量小胶质细胞，但这两个特征在发病机制上是否相关尚不清楚。因此，我们使用博伊登小室试验研究了小鼠小胶质细胞对GL261小鼠胶质瘤细胞运动性的影响。在有小胶质细胞存在的情况下，胶质瘤细胞迁移更早出现，48小时后，与没有小胶质细胞的培养相比，迁移率高出三倍。这种效应是由小胶质细胞释放的物质介导的，因为在小胶质细胞条件培养基中观察到了类似的效应，并且这种效应是小胶质细胞特有的，因为少突胶质细胞和内皮细胞对胶质瘤细胞迁移的刺激较弱。小胶质细胞激活物质（GM-CSF、LPS）导致运动性进一步增加。这些数据支持这样一种观点，即弥漫性胶质肿瘤中小胶质细胞的积累不仅代表对组织损伤的非特异性反应，还反映了这些细胞参与支持和促进星形细胞瘤细胞的侵袭表型。
