Fung Eric T, Enderwick Cynthia
Ciphergen Biosystems, Fremont, CA 94555, USA.
Biotechniques. 2002 Mar;Suppl:34-8, 40-1.
ProteinChip technology, a suite of analytical tools that includes retentate chromatography, on-chip protein characterization, and multivariate analysis, allows researchers to examine patterns ofprotein expression and modification. Based on the surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) approach, ProteinChip technology has been pioneered by researchers at Ciphergen Biosystems (Fremont, CA, USA), as well as by users of Ciphergen's commercial embodiment of this technology the ProteinChip Biomarker System. This report will begin with a background of the technology and describe its applications in clinical proteomics and will then conclude with a discussion of tools and strategies to mine the large amounts of data generated during the course of a typical clinical proteomics study.
蛋白质芯片技术是一套分析工具，包括截留物色谱法、芯片上蛋白质表征和多变量分析，它使研究人员能够检测蛋白质表达和修饰模式。基于表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）方法，蛋白质芯片技术由Ciphergen生物系统公司（美国加利福尼亚州弗里蒙特）的研究人员以及Ciphergen该技术商业版本蛋白质芯片生物标志物系统的用户率先开发。本报告将首先介绍该技术的背景并描述其在临床蛋白质组学中的应用，然后讨论挖掘典型临床蛋白质组学研究过程中产生的大量数据的工具和策略。
