Di Girolamo Francesco, Lante Isabella, Muraca Maurizio, Putignani Lorenza
Laboratory Medicine, Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4, 00165, Rome, Italy;
Laboratory Medicine, San Camillo Hospital, Viale Vittorio Veneto 18, 31100, Treviso, Italy;
Curr Org Chem. 2013 Dec;17(23):2891-2905. doi: 10.2174/1385272817888131118162725.
Mass spectrometry (MS) is one of the key analytical technology on which the emerging ''-omics'' approaches are based. It may provide detection and quantization of thousands of proteins and biologically active metabolites from a tissue, body fluid or cell culture working in a ''global'' or ''targeted'' manner, down to ultra-trace levels. It can be expected that the high performance of MS technology, coupled to routine data handling, will soon bring fruit in the request for a better understanding of human diseases, leading to new molecular biomarkers, hence affecting drug targets and therapies. In this review, we focus on the main advances in the MS technologies, influencing genomics, transcriptomics, proteomics, lipidomics and metabolomics fields, up to the most recent MS applications to meta-omic studies.
质谱（MS）是新兴的“组学”方法所基于的关键分析技术之一。它能够以“全局”或“靶向”方式，对来自组织、体液或细胞培养物中的数千种蛋白质和生物活性代谢物进行检测和定量，低至超痕量水平。可以预期，质谱技术的高性能与常规数据处理相结合，将很快在更好地理解人类疾病的需求方面取得成果，从而产生新的分子生物标志物，进而影响药物靶点和治疗方法。在本综述中，我们重点关注质谱技术在基因组学、转录组学、蛋白质组学、脂质组学和代谢组学领域的主要进展，直至质谱在元组学研究中的最新应用。
