Tayou Tagny C, Kouam L, Mbanya D
Laboratoire d'hématologie et banque de sang, Centre hospitalier et universitaire de Yaoundé, Cameroun.
Ann Biol Clin (Paris). 2008 Jan-Feb;66(1):90-4. doi: 10.1684/abc.2008.0195.
The performance of the HemoCue apparatus in the diagnosis of anaemia in pregnancy was evaluated using the HemoCue Hb 301 and the HemoCue Hb 201+ apparatus. The HemoCue Hb 201 + was considered as the reference method in this study. Two hundred and thirty six pregnant women attending the University Teaching Hospital in Yaoundé (Cameroon) were included in the analysis. Anaemia was defined as haemoglobin < 11 g/dL in pregnant women during the first and the third trimester of pregnancy and as <10. 5 g/dL during the second trimester of pregnancy. With the reference method, anaemia was present in 17.7% of pregnant women compared to 11% using the HemoCue 301 method. The sensitivity, specificity, positive and negative predictive values for the HemoCue 301 method were respectively 61.9%, 100%, 100% and 92.3%. A strong correlation was shown with a Pearson's coefficient of 0.98. Using the Bland and Altman method for assessing agreement between two methods, the 95% (+/- 2SD) distribution of Hb levels about the mean was not much spread (0.06 - 0.52) though it was positive, with slightly higher Hb levels in the HemoCue Hb 301 method, compared to the reference method. Considering the study of the precision, coefficient of variation of the two methods were similar. It may be concluded that the HemoCue Hb 301 method compared to the HemoCue Hb 201+ method has acceptable sensitivity and is very specific in the diagnosis of anaemia in the Cameroonian pregnant woman. Although it gives slightly higher values, it is precise and exact in measuring Hb levels.
使用HemoCue Hb 301和HemoCue Hb 201+仪器评估了HemoCue仪器在诊断妊娠贫血中的性能。在本研究中，HemoCue Hb 201+被视为参考方法。对236名在雅温得（喀麦隆）大学教学医院就诊的孕妇进行了分析。贫血的定义为：妊娠第一和第三孕期孕妇血红蛋白<11 g/dL，妊娠第二孕期血红蛋白<10.5 g/dL。采用参考方法，17.7%的孕妇存在贫血，而使用HemoCue 301方法的这一比例为11%。HemoCue 301方法的灵敏度、特异性、阳性预测值和阴性预测值分别为61.9%、100%、100%和92.3%。Pearson系数为0.98，显示出很强的相关性。使用Bland和Altman方法评估两种方法之间的一致性，虽然Hb水平相对于平均值的95%（±2SD）分布呈正值且分布范围不大（0.06 - 0.52），但与参考方法相比，HemoCue Hb 301方法的Hb水平略高。考虑到精密度研究，两种方法的变异系数相似。可以得出结论，与HemoCue Hb 201+方法相比，HemoCue Hb 301方法在诊断喀麦隆孕妇贫血方面具有可接受的灵敏度且非常特异。尽管它给出的值略高，但在测量Hb水平方面精确且准确。
