Kuddus Ruhul H, Kuddus Nayema N, Dvorchik Igor
Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh School of Medicine, PA 15213, USA.
Genome. 2002 Feb;45(1):8-12. doi: 10.1139/g01-115.
Random amplified polymorphic DNA (RAPD) analysis is a valuable tool in studying inter- and intra-specific genetic variations, patterns of gene expression, and for the identification of specific genes using nearly isogenic variants. Here we used RAPD analysis to study the genetic variation in Ginkgo biloba grown in the eastern United States. Our results support the evidence that Southern blot hybridization of RAPD using probes made from cloned DNA fragments allows a more accurate analysis of the RAPD pattern than dye-stained gels or Southern blot hybridization of RAPD blots using probes made from purified PCR products. Using these techniques, we observed a high degree of relatedness among plants grown in certain localities although significant genetic variation may exist in the species, and could be a possible explanation for the observed variations in the efficacy of medications derived from G. biloba extract.
随机扩增多态性DNA（RAPD）分析是研究种间和种内遗传变异、基因表达模式以及使用近等基因变体鉴定特定基因的宝贵工具。在此，我们使用RAPD分析来研究生长在美国东部的银杏的遗传变异。我们的结果支持这样的证据，即使用由克隆DNA片段制成的探针进行RAPD的Southern印迹杂交，比使用染料染色凝胶或使用由纯化PCR产物制成的探针进行RAPD印迹的Southern印迹杂交，能更准确地分析RAPD模式。使用这些技术，我们观察到在某些地区生长的植物之间具有高度的相关性，尽管该物种可能存在显著的遗传变异，这可能是从银杏叶提取物衍生的药物疗效观察到的变异的一个可能解释。
