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利用RAPD标记评估中国银杏(银杏科)种群的遗传多样性。

Assessing genetic diversity of Ginkgo biloba l. (Ginkgoaceae) populations from China by RAPD markers.

作者信息

Fan Xiao-Xia, Shen Lang, Zhang Xin, Chen Xiao-Yong, Fu Cheng-Xin

机构信息

Department of Environmental Sciences, East China Normal University, Shanghai 200062, People's Republic of China.

出版信息

Biochem Genet. 2004 Aug;42(7-8):269-78. doi: 10.1023/b:bigi.0000034431.15308.57.

DOI:10.1023/b:bigi.0000034431.15308.57
PMID:15487590
Abstract

Genetic diversity and differentiation of nine populations of Ginkgo biloba L. (Ginkgoaceae)from China were evaluated using RAPD. Of 47 clear and repeatable RAPD bands, 46 were polymorphic (overall polymorphism = 97.9%). A ranged from 1.57 to 1.83 with a mean of 1.75. Mean He was 0.3159 (0.2429-0.3603). The Shannon index ranged from 0.3432 to 0.5119 with a mean of 0.4489. The GST was 0.1609 and AMOVA analysis indicated 89% of the variation within populations. UPGMA clustered the 9 populations into two groups: one containing only population JF and the other including 8 populations. Genetic diversity and differentiation of Chinese populations were higher than those of Korean and North American populations, which are ultimately descended from China, as reported previously.

摘要

利用随机扩增多态性DNA(RAPD)技术对来自中国的9个银杏种群的遗传多样性和分化进行了评估。在47条清晰且可重复的RAPD条带中,46条具有多态性(总体多态性 = 97.9%)。A值范围为1.57至1.83,平均值为1.75。平均期望杂合度(He)为0.3159(0.2429 - 0.3603)。香农指数范围为0.3432至0.5119,平均值为0.4489。基因分化系数(GST)为0.1609,分子方差分析(AMOVA)表明89%的变异存在于种群内部。非加权组平均法(UPGMA)将9个种群聚为两组:一组仅包含JF种群,另一组包含8个种群。如先前报道,中国种群的遗传多样性和分化高于韩国和北美种群,而后两者最终起源于中国。

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