Fan Xiao-Xia, Shen Lang, Zhang Xin, Chen Xiao-Yong, Fu Cheng-Xin
Department of Environmental Sciences, East China Normal University, Shanghai 200062, People's Republic of China.
Biochem Genet. 2004 Aug;42(7-8):269-78. doi: 10.1023/b:bigi.0000034431.15308.57.
Genetic diversity and differentiation of nine populations of Ginkgo biloba L. (Ginkgoaceae)from China were evaluated using RAPD. Of 47 clear and repeatable RAPD bands, 46 were polymorphic (overall polymorphism = 97.9%). A ranged from 1.57 to 1.83 with a mean of 1.75. Mean He was 0.3159 (0.2429-0.3603). The Shannon index ranged from 0.3432 to 0.5119 with a mean of 0.4489. The GST was 0.1609 and AMOVA analysis indicated 89% of the variation within populations. UPGMA clustered the 9 populations into two groups: one containing only population JF and the other including 8 populations. Genetic diversity and differentiation of Chinese populations were higher than those of Korean and North American populations, which are ultimately descended from China, as reported previously.
利用随机扩增多态性DNA(RAPD)技术对来自中国的9个银杏种群的遗传多样性和分化进行了评估。在47条清晰且可重复的RAPD条带中,46条具有多态性(总体多态性 = 97.9%)。A值范围为1.57至1.83,平均值为1.75。平均期望杂合度(He)为0.3159(0.2429 - 0.3603)。香农指数范围为0.3432至0.5119,平均值为0.4489。基因分化系数(GST)为0.1609,分子方差分析(AMOVA)表明89%的变异存在于种群内部。非加权组平均法(UPGMA)将9个种群聚为两组:一组仅包含JF种群,另一组包含8个种群。如先前报道,中国种群的遗传多样性和分化高于韩国和北美种群,而后两者最终起源于中国。