Hart R W, Setlow R B
Basic Life Sci. 1975;5B:719-24. doi: 10.1007/978-1-4684-2898-8_47.
A specific test for the biological role of UV-induced pyrimidine dimers in DNA is photoreactivation (PR). Fish contain large amounts of the PR enzyme. Portions of cell suspensions of tissue from various organs of the fish Poecilia formosa were exposed to UV radiation (254 nm), then injected into isogenic recipients. An incident fluence of 20 J/m2 resulted in 10% of the fish with large granulomas and 100% with thyroid carcinomas. If the irradiated cell suspension was illuminated with PR light before injection, the yields of both types of lesion were reduced approximately 10-Fold. If the PR light was given before the UV exposure, there was no reduction in the numbers of growths. These experiments show that pyrimidine dimers in DNA can lead to neoplastic transformation.
对紫外线诱导的DNA嘧啶二聚体的生物学作用进行的一项特定测试是光复活作用（PR）。鱼类含有大量的PR酶。将来自墨西哥食蚊鱼不同器官组织的细胞悬液部分暴露于紫外线辐射（254纳米），然后注射到同基因受体中。20 J/m² 的入射通量导致10%的鱼出现大的肉芽肿，100%的鱼出现甲状腺癌。如果在注射前用PR光照射辐照过的细胞悬液，两种类型损伤的发生率都降低了约10倍。如果在紫外线照射前给予PR光，则生长物数量没有减少。这些实验表明，DNA中的嘧啶二聚体可导致肿瘤转化。
