高等植物中卫星脱氧核糖核酸的复杂性。
The complexity of satellite deoxyribonucleic acid in a higher plant.
作者信息
Sinclair J, Wells R, Deumling B, Ingle J
出版信息
Biochem J. 1975 Jul;149(1):31-8. doi: 10.1042/bj1490031.
Abstract
Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.
摘要
来自甜瓜（黄瓜）的纯化卫星DNA经热变性和复性研究表明至少含有两个组分。部分复性后分离出两个组分，一个快速复性组分，其复杂度与小鼠卫星DNA相似，另一个组分的复杂度则高6000倍。两个组分都能非常精确地复性，表明序列差异极小。在Ag⁺/Cs₂SO₄梯度中对纯化的卫星DNA进行离心，分离出两个主要组分和几个次要组分。这两个主要组分在快速或慢速复性序列上仅有轻微富集。
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