Evgen'ev M, Levin A, Lozovskaya E
Mol Gen Genet. 1979 Oct 3;176(2):275-80. doi: 10.1007/BF00273222.
The effect of a ts-mutation belonging to the cell-lethal type on protein and RNA synthesis under heat shock conditions has been investigated. The mutation studied, l(l)ts-403, localized in the X-chromosome has significant effect on the kinetics of protein synthesis in salivary gland subjected to heat treatment under in vivo and in vitro conditions. The animals homozygous for l(l)ts-403 are characterized by a rapid drop of label incorporation into heat shock proteins. It is shown that the mutation affects not only the kinetics of heat-shock proteins synthesis but their electrophoretic pattern as well. The experiments performed enable us to conclude that the presumptive regulatory gene realized its action at the level of transcription of heat shock loci.
研究了属于细胞致死型的温度敏感突变在热休克条件下对蛋白质和RNA合成的影响。所研究的突变l(l)ts - 403定位在X染色体上，在体内和体外条件下，对经受热处理的唾液腺中蛋白质合成的动力学有显著影响。l(l)ts - 403纯合的动物表现为热休克蛋白中标记掺入迅速下降。结果表明，该突变不仅影响热休克蛋白合成的动力学，还影响其电泳图谱。所进行的实验使我们能够得出结论，推定的调控基因在热休克基因座的转录水平发挥作用。
