García C C, Rosso M L, Bertoni M D, Maier M S, Damonte E B
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Planta Med. 2002 Mar;68(3):209-12. doi: 10.1055/s-2002-23134.
Four macrocyclic trichothecenes, roridin A, roridin E, verrucarin A and verrucarin J, produced by the hypocrealean epibiont of Baccharis coridifolia, were evaluated for their inhibitory activity against the arenavirus Junin (JUNV), the etiological agent of Argentine hemorrhagic fever. The trichothecenes achieved a dose-dependent inhibition of JUNV multiplication at concentrations not affecting cell viability. The 50 % inhibitory concentration (IC50) values determined by a virus yield inhibition assay were in the range 1.2 - 4.9 ng/ml. The most active compound was verrucarin J which reduced JUNV yield more than 2 log units and had a similar effect against the arenavirus Tacaribe. The trichothecenes lacked virucidal effects on JUNV virions. From time of addition and removal experiments, it can be concluded that verrucarin J inhibited a late stage in the replicative cycle of JUNV, after 5 h of adsorption.
对由科氏酒神菊（Baccharis coridifolia）的肉座菌外生菌产生的四种大环单端孢霉烯族毒素——A组棒曲霉素、E组棒曲霉素、A组疣孢菌素和J组疣孢菌素，评估了它们对阿根廷出血热的病原体——胡宁沙粒病毒（JUNV）的抑制活性。在不影响细胞活力的浓度下，单端孢霉烯族毒素实现了对JUNV增殖的剂量依赖性抑制。通过病毒产量抑制试验确定的50%抑制浓度（IC50）值在1.2 - 4.9 ng/ml范围内。活性最强的化合物是J组疣孢菌素，它使JUNV产量降低超过2个对数单位，并且对塔卡里贝沙粒病毒有类似作用。单端孢霉烯族毒素对JUNV病毒粒子没有杀病毒作用。从添加和去除时间实验可以得出结论，J组疣孢菌素在吸附5小时后抑制了JUNV复制周期的后期阶段。
