Schulman H M
Biochim Biophys Acta. 1975 Dec 4;414(2):161-6. doi: 10.1016/0005-2787(75)90219-1.
The synthesis of heme and globin in rabbit reticulocytes was compared at 35 and 25 degrees C. The lower temperature decreased heme synthesis significantly more than globin synthesis and resulted in a much greater accumulation of globin dimers. After 16 h of incubation in the absence of iron, globin synthesis in reticulocytes which were at 35 degrees C could not be stimulated by iron, whereas cells which were at 25 degrees C responded with nearly control levels of globin synthesis. Since the formation of the hemin-controlled translational repressor in reticulocyte lysates is also decreased much more than protein synthesis at reduced temperature the results provide evidence for a physiological role for the translational repressor in controlling globin synthesis in reticulocytes.
在35摄氏度和25摄氏度下比较了兔网织红细胞中血红素和珠蛋白的合成。较低温度下血红素合成的减少幅度明显大于珠蛋白合成，导致珠蛋白二聚体积累得更多。在无铁条件下孵育16小时后，35摄氏度的网织红细胞中的珠蛋白合成不能被铁刺激，而25摄氏度的细胞珠蛋白合成水平接近对照水平。由于在较低温度下网织红细胞裂解物中血红素控制的翻译阻遏物的形成减少幅度也远大于蛋白质合成，这些结果为翻译阻遏物在控制网织红细胞中珠蛋白合成方面的生理作用提供了证据。
