Strasberg P M, Freeman K B
Can J Biochem. 1975 Nov;53(11):1150-7. doi: 10.1139/o75-160.
Proteins of the rat liver mitochondrial matrix have been separated into anionic (acidic), cationic(basic), and neutral groups by electrophoresis. These groups represent 69, 8, and 23% of the total matrix protein, respectively, compared to 69, 21, and 10% for the cytosol protein. The acidic nature of the mitochondrial matrix proteins has been confirmed by cellulose ion-exhange chromatography, isoelectric focusing in sucrose gradients, and amino acid analysis. The anionic, cationic, and neutral matrix proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis into 18, 6, and 5 bands, respectively, compared to 22 bands for the total fraction. The significance of the charge properties of these proteins in terms of mitochondrial biogenesis is discussed.
大鼠肝脏线粒体基质蛋白已通过电泳被分离为阴离子（酸性）、阳离子（碱性）和中性组。这些组分别占总基质蛋白的69%、8%和23%，相比之下，胞质溶胶蛋白的这一比例分别为69%、21%和10%。线粒体基质蛋白的酸性性质已通过纤维素离子交换色谱法、蔗糖梯度等电聚焦法和氨基酸分析得到证实。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳将阴离子、阳离子和中性基质蛋白分别分离为18条、6条和5条带，而总组分则分离为22条带。本文讨论了这些蛋白质的电荷特性在线粒体生物发生方面的意义。
