Scaletsky Isabel C A, Fabbricotti Sandra H, Aranda Katia R, Morais Mauro B, Fagundes-Neto Ulysses
Department of Microbiology, Immunology, and Parasitology, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, SP, Brazil CEP 04023-062.
J Clin Microbiol. 2002 Apr;40(4):1254-8. doi: 10.1128/JCM.40.4.1254-1258.2002.
The correlation of the different adherence patterns with DNA probes and PCR primers for the identification of Escherichia coli was analyzed in isolates from children, less than 2 years of age with or without diarrhea, from different regions of Brazil. A total of 1,428 isolates obtained from 338 patients and 322 control children were studied. The enteropathogenic E. coli (EPEC) adherence factor (EAF) probe was shown to be as good as the HEp-2 adhesion assay for the detection of typical EPEC strains. The DNA probes used to detect diffusely adhering E. coli and enteroaggregative E. coli (EAEC) showed low sensitivities (64 and 50%, respectively), and the best method of identifying these organisms in clinical research remains the HEp-2 adherence assay. The "bundle-forming pilus" (BFP) and the EAEC PCR assays could be used instead of the DNA probes as a screening method for typical EPEC and EAEC carrying the EAEC probe sequence in the clinical laboratory. In our study, only typical EPEC strains that carried EAF and BFP were associated with acute diarrhea.
对来自巴西不同地区年龄小于2岁、有或无腹泻的儿童分离株，分析了不同黏附模式与用于鉴定大肠杆菌的DNA探针和PCR引物之间的相关性。共研究了从338例患者和322名对照儿童中获得的1428株分离株。结果显示，肠致病性大肠杆菌（EPEC）黏附因子（EAF）探针在检测典型EPEC菌株方面与HEp-2黏附试验效果相当。用于检测弥漫性黏附大肠杆菌和肠聚集性大肠杆菌（EAEC）的DNA探针敏感性较低（分别为64%和50%），临床研究中鉴定这些菌的最佳方法仍是HEp-2黏附试验。“束状菌毛”（BFP）和EAEC PCR检测可替代DNA探针，作为临床实验室筛查携带EAEC探针序列的典型EPEC和EAEC的方法。在我们的研究中，只有携带EAF和BFP的典型EPEC菌株与急性腹泻有关。
