Songserm Th, Zekarias B, van Roozelaar D J, Kok R S, Pol J M A, Pijpers A A, ter Huurne A A H M
ID-Lelystad BV, Institute for Animal Science and Health, The Netherlands.
Avian Dis. 2002 Jan-Mar;46(1):87-94. doi: 10.1637/0005-2086(2002)046[0087:EROMSW]2.0.CO;2.
Attempts to reproduce malabsorption syndrome (MAS) by oral inoculation with several different combinations including intestinal homogenate, reovirus, and hemolytic Escherichia coli obtained from MAS-affected chickens and intestinal homogenate from healthy chickens (healthy homogenate) were performed in 1-day-old specific-pathogen-free (SPF) broilers. The MAS homogenate, serving as a positive control, induced weight gain depression and intestinal lesions such as cystic crypts of Lieberkuhn, villus atrophy, and lymphoid and/or granulocytic infiltration. The healthy homogenate, the formalin-treated MAS homogenate, the formalin-treated healthy homogenate, and phosphate-buffered saline caused neither weight gain depression nor intestinal lesions. We were able to reproduce both weight gain depression and intestinal lesions by inoculation of reovirus either combined with the formalin-treated MAS homogenate or combined with healthy homogenate. Surprisingly, when hemolytic E. coli was added to the combination of reovirus with formalin-treated MAS homogenate, this did not cause weight gain depression although this combination caused the described intestinal lesions. Identical results were obtained with the combination of formalin-treated MAS homogenate with hemolytic E coli or the combination of reovirus with hemolytic E. coli. The intestinal lesions were more severe and developed faster by combinations including reovirus and formalin-treated MAS homogenate. This study indicates that a combination of enteropathogenic reovirus with other agents or substances that are present in an intestinal homogenate from MAS-affected and healthy chickens can induce MAS in SPF broilers. Escherichia coli is not essential for induction of weight gain depression but can play a role in development of intestinal lesions. Furthermore, intestinal lesions alone will not always result in weight gain depression.
在1日龄无特定病原体（SPF）肉鸡中，尝试通过口服接种几种不同组合来复制吸收不良综合征（MAS），这些组合包括从患MAS的鸡获得的肠道匀浆、呼肠孤病毒和溶血性大肠杆菌，以及来自健康鸡的肠道匀浆（健康匀浆）。作为阳性对照的MAS匀浆导致体重增加受抑制，并出现肠道病变，如利伯kühn氏隐窝囊肿、绒毛萎缩以及淋巴细胞和/或粒细胞浸润。健康匀浆、经福尔马林处理的MAS匀浆、经福尔马林处理的健康匀浆和磷酸盐缓冲盐水既未导致体重增加受抑制，也未引起肠道病变。通过接种与经福尔马林处理的MAS匀浆联合或与健康匀浆联合的呼肠孤病毒，我们能够复制体重增加受抑制和肠道病变。令人惊讶的是，当将溶血性大肠杆菌添加到呼肠孤病毒与经福尔马林处理的MAS匀浆的组合中时，尽管这种组合导致了所述的肠道病变，但并未引起体重增加受抑制。用经福尔马林处理的MAS匀浆与溶血性大肠杆菌的组合或呼肠孤病毒与溶血性大肠杆菌的组合获得了相同的结果。包括呼肠孤病毒和经福尔马林处理的MAS匀浆的组合导致的肠道病变更严重且发展更快。这项研究表明，肠道致病性呼肠孤病毒与来自患MAS和健康鸡的肠道匀浆中存在的其他病原体或物质的组合可在SPF肉鸡中诱导MAS。大肠杆菌对于诱导体重增加受抑制并非必不可少，但可在肠道病变的发展中起作用。此外，单独的肠道病变并不总是会导致体重增加受抑制。
