Stern M P, Conrad F
Clin Chim Acta. 1975 Nov 15;65(1):29-37. doi: 10.1016/0009-8981(75)90331-9.
Current methods for direct determination of fat cell size are optical and non-automated. They are thus tedious, but have the advantage of providing estimates of the variance of a cell size distribution, as well as a measure of the mean cell size. An indirect method based on counting the number of osmium-fixed fat cells derived from a known wet weight of adipose tissue is also available. This method is automated and thus rapid, but does not provide information about the variance of the cell size distribution. In the present paper we describe a direct, automated method of fat cell sizing that provides estimates for both mean cell size and the variance of the cell size distribution. The correlation between our method and in indirect method based on counting osmium-fixed cells was 0.96. Transformation of the volume distribution to diameters indicated that cell diameters appeared to be normally distributed, confirming the observations of others.
目前直接测定脂肪细胞大小的方法是光学方法且非自动化。因此这些方法很繁琐，但具有能提供细胞大小分布方差估计值以及平均细胞大小测量值的优点。一种基于计算从已知湿重脂肪组织中获得的锇固定脂肪细胞数量的间接方法也可行。该方法是自动化的，因而速度快，但不提供关于细胞大小分布方差的信息。在本文中，我们描述了一种直接的、自动化的脂肪细胞大小测定方法，该方法能提供平均细胞大小和细胞大小分布方差的估计值。我们的方法与基于计算锇固定细胞的间接方法之间的相关性为0.96。将体积分布转换为直径表明细胞直径似乎呈正态分布，这证实了其他人的观察结果。
