Richters C D, Mayen I, Havenith C E G, Beelen R H J, Kamperdijk E W A
Department of Molecular Cell Biology, Faculty of Medicine, VUMC, Amsterdam, The Netherlands.
J Leukoc Biol. 2002 Apr;71(4):582-7.
Dendritic cells (DC) are the most potent antigen-presenting cells and are therefore useful to induce immune responses against tumor cells in patients. DC can be generated in vitro from monocytes using GM-CSF and IL-4, the so-called monocyte-derived DC (MoDC). To achieve antitumor responses, MoDC must be able to migrate to the draining lymph nodes after injection to induce cytotoxic T cells. Therefore, we studied migration of MoDC in a rat model. Functional rat MoDC were generated from PVG-RT7B rats and injected subcutaneously into PVG rats. These rat strains differ only at one epitope of the leukocyte-common antigen, which can be recognized by the antibody His 41. The advantage is that migrated cells can be detected in the draining lymph nodes by staining sections with His 41+; thus, migration is not influenced by labeling procedures. Rat MoDC migrated to the T-cell areas of the draining lymph nodes, just as isolated Langerhans cells or spleen DC do. In contrast, monocytes also migrated to the B-cell areas and the medulla.
树突状细胞（DC）是最有效的抗原呈递细胞，因此有助于诱导患者针对肿瘤细胞的免疫反应。使用粒细胞巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-4（IL-4），可以在体外从单核细胞生成DC，即所谓的单核细胞衍生DC（MoDC）。为了实现抗肿瘤反应，MoDC在注射后必须能够迁移至引流淋巴结以诱导细胞毒性T细胞。因此，我们在大鼠模型中研究了MoDC的迁移。功能性大鼠MoDC由PVG-RT7B大鼠生成，并皮下注射到PVG大鼠体内。这些大鼠品系仅在白细胞共同抗原的一个表位上存在差异，该表位可被抗体His 41识别。其优势在于，通过用His 41+对切片进行染色，可在引流淋巴结中检测到迁移的细胞；因此，迁移不受标记程序的影响。大鼠MoDC迁移至引流淋巴结的T细胞区域，就如同分离出的朗格汉斯细胞或脾脏DC一样。相比之下，单核细胞也迁移至B细胞区域和髓质。
