Lin Chun-Cheng, Yeh Yi-Chun, Yang Chan-Yi, Chen Chan-Long, Chen Gee-Fong, Chen Chia-Chun, Wu Yi-Chun
Institute of Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan.
J Am Chem Soc. 2002 Apr 10;124(14):3508-9. doi: 10.1021/ja0200903.
The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.
本文报道了甘露糖包被的金纳米颗粒（m-AuNP）的合成、表征及其生物学应用。m-AuNP在宽离子强度和pH范围的介质中分散良好且非常稳定，无聚集现象。利用透射电子显微镜证明了m-AuNP与细菌1型菌毛的甘露糖粘附素FimH的选择性结合。与游离甘露糖的竞争试验表明，m-AuNP比游离甘露糖更能有效地结合FimH。这项工作表明，碳水化合物连接的纳米颗粒可作为生物系统中一种有效的亲和标记物和多配体载体。
