Slate D L, Shulman L, Lawrence J B, Revel M, Ruddle F H
J Virol. 1978 Jan;25(1):319-25. doi: 10.1128/JVI.25.1.319-325.1978.
Human/mouse somatic cell hybrids with chromosome 21 as the only detectable human genetic material were sensitive to both human leukocyte and fibroblast interferons. The presence of additional human chromosomes decreased the amount of interferon needed to attain a given level of virus resistance. Decreased cytopathic effects, decreased virus yields, and the appearance of a specific phosphorylated protein associated with interferon treatment were all observed in hybrids maintaining only human chromosome 21. The phosphorylated protein found in extracts of these human interferon-treated hybrid cells was of mouse origin.
以21号染色体作为唯一可检测到的人类遗传物质的人/鼠体细胞杂种，对人白细胞干扰素和成纤维细胞干扰素均敏感。额外人类染色体的存在减少了达到给定病毒抗性水平所需的干扰素量。在仅保留人类21号染色体的杂种细胞中，均观察到细胞病变效应降低、病毒产量降低以及与干扰素处理相关的一种特定磷酸化蛋白的出现。在这些经人干扰素处理的杂种细胞提取物中发现的磷酸化蛋白源自小鼠。
