Meager A, Graves H E, Walker J R, Burke D C, Swallow D M, Westerveld A
J Gen Virol. 1979 Nov;45(2):309-21. doi: 10.1099/0022-1317-45-2-309.
Forty-two primary human-mouse cell hybrids, derived in two separate experiments, were treated with Newcastle disease virus (NDV): eight hybrids were found to produce human interferon and this was shown in every case to be predominantly of the fibroblast type. An extensive analysis was made in terms of karyotype and marker enzymes on all the eight hybrids producing interferon and also on five hybrids which did not produce interferon, five randomly selected hybrids and eleven subclones resistant to diphtheria toxin. The results suggest that, contrary to previous reports, a gene on chromosome 5 is not involved in production of human interferon. Its production was however correlated with the presence of chromosome 9 in the hybrids. Analyses of two sets of human-Chinese hamster hybrid subclones from two different crosses were also consistent with the assignment of a human interferon gene to chromosome 9.
在两个独立实验中获得的42个人类-小鼠细胞杂种用新城疫病毒（NDV）进行处理：发现8个杂种产生人干扰素，并且在每种情况下都表明主要是成纤维细胞型。对所有8个产生干扰素的杂种以及5个不产生干扰素的杂种、5个随机选择的杂种和11个对白喉毒素有抗性的亚克隆进行了核型和标记酶方面的广泛分析。结果表明，与先前的报道相反，5号染色体上的一个基因不参与人干扰素的产生。然而，其产生与杂种中9号染色体的存在相关。对来自两个不同杂交的两组人类-中国仓鼠杂种亚克隆的分析也与将人干扰素基因定位于9号染色体一致。
