胰腺癌患者血清中的自身抗体将重组因子Rad51鉴定为一种肿瘤相关抗原。
Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen.
作者信息
Maacke H, Hundertmark C, Miska S, Voss M, Kalthoff H, Stürzbecher H-W
机构信息
Present address: Clinic for Internal Medicine II, Friedrich-Schiller University Jena, Erlanger Allee 101, 07743 Jena, Germany.
出版信息
J Cancer Res Clin Oncol. 2002 Apr;128(4):219-22. doi: 10.1007/s00432-001-0321-2. Epub 2002 Feb 6.
PURPOSE
The recombination factor Rad51 is highly expressed in human pancreatic adenocarcinoma. In this study we asked whether high-level expression of Rad51 antigen stimulates a B-cell response leading to Rad51-specific autoantibodies in human pancreatic cancer patients.
METHODS
Sera of patients suffering from pancreatic cancer (57) as well as sera of healthy donors (86) were screened for Rad51 autoantibodies by Western-blot analysis. Rad51 over-expressing cell lines were used as antigen source.
RESULTS
Four out of 57 (7%) sera tested were found positive for Rad51 autoantibodies of IgG subclass, while all 86 control sera were negative.
CONCLUSION
This observation identifies Rad51 as a tumour-associated antigen in pancreatic adenocarcinoma. Since high-level expression of Rad51 is restricted to tumour cells, Rad51 is also a tumour-specific antigen. Further analyses should reveal whether Rad51 might also function as a tumour-specific transplantation antigen (TSTA) and whether it might serve as a target for new immunotherapeutical approaches.
目的
重组因子Rad51在人类胰腺腺癌中高表达。在本研究中，我们探讨Rad51抗原的高表达是否会刺激B细胞反应，从而导致人类胰腺癌患者产生Rad51特异性自身抗体。
方法
通过蛋白质免疫印迹分析筛选胰腺癌患者（57例）以及健康供者（86例）血清中的Rad51自身抗体。将过表达Rad51的细胞系用作抗原来源。
结果
在检测的57份血清中，有4份（7%）IgG亚类的Rad51自身抗体呈阳性，而所有86份对照血清均为阴性。
结论
该观察结果确定Rad51为胰腺腺癌中的一种肿瘤相关抗原。由于Rad51的高表达仅限于肿瘤细胞，因此Rad51也是一种肿瘤特异性抗原。进一步分析应揭示Rad51是否也可能作为肿瘤特异性移植抗原（TSTA）发挥作用，以及它是否可作为新免疫治疗方法的靶点。
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