Radov L A, Sussdorf D H, McCann R L
Immunology. 1975 Dec;29(6):977-88.
In nude mice back-crossed a minimum of five times to BALB/c, solid thymus grafts from C57Bl donors 3 days of age or younger restored both the humoral immune response against sheep erythrocytes and cellular immunity as tested by rejection of CBA skin grafts. Donor thymus placed under the renal capsule at a dose of 0-5 mg/g of recipient resulted in normal humoral immunity, while a minimum dose of 1-5 mg/g was required to reconstitute cellular competence. None of the various amounts of allogeneic thymus tissue transplanted affected the immunological status of nude recipients when grafts were obtained from donors 4 days of age or older. Histological findings correlated with the humoral and cellular responses observed. In nudes grafted with neonatal tissue, the thymus implant proliferated and developed normal architecture. The density of lymphocytes in thymus-dependent regions of peripheral lymphoid organs was near normal. On the other hand, most grafts from older (3-week-old) donors were resorbed by 90 days after implantation. In a number of cases, however, Russell bodies and numerous blast and plasma cells were seen in the graft site. Our observations suggest a possible cytotoxic rejection of implants from older allogeneic donors, while the survival and restorative capacity of transplants from 3-day-old or younger donors may have been due to a tolerogenic effect of the graft on the nude recipient.
在与BALB/c进行至少五次回交的裸鼠中，来自3日龄及以下C57Bl供体的实体胸腺移植恢复了针对绵羊红细胞的体液免疫反应以及通过CBA皮肤移植排斥反应测试的细胞免疫。以0 - 5毫克/克受体的剂量将供体胸腺置于肾被膜下可导致正常的体液免疫，而重建细胞能力则需要至少1 - 5毫克/克的剂量。当从4日龄及以上的供体获取移植组织时，移植的各种量的同种异体胸腺组织均未影响裸鼠受体的免疫状态。组织学发现与观察到的体液和细胞反应相关。在移植了新生组织的裸鼠中，胸腺植入物增殖并发育出正常结构。外周淋巴器官胸腺依赖区的淋巴细胞密度接近正常。另一方面，大多数来自较老（3周龄）供体的移植组织在植入后90天被吸收。然而，在许多情况下，在移植部位可见拉塞尔小体以及大量的母细胞和浆细胞。我们的观察结果表明，来自较老同种异体供体的植入物可能存在细胞毒性排斥反应，而来自3日龄及以下供体的移植组织的存活和恢复能力可能归因于移植对裸鼠受体的致耐受性作用。
