Siminoff P
Infect Immun. 1975 Nov;12(5):1051-7. doi: 10.1128/iai.12.5.1051-1057.1975.
BL-20803, a low-molecular-weight compound, although able to elicit circulating interferon in the mouse, failed to protect cultured cell lines in vitro from infection by interferon-sensitive viruses. Of the tissues analyzed for interferon content after oral administration of the drug to mice, spleen and lung contained the largest amounts of the virus inhibitor. Spleen cells from such dosed animals when isolated into in vitro cultures elaborated small amounts of interferon into the culture medium. The time sequence of acquisition by spleen cells of the ability to produce interferon closely correlated with the kinetics of development of the circulating interferon response in the intact mouse. When spleen cells were separated on the basis of adherence or nonadherence to a plastic surface, the bulk of the interferon activity was found to be associated with the adherent cells. Upon exposure to BL-20803 in cell culture, adherent cells and, to a lesser extent, nonadherent cells from untreated mice were stimulated to produce interferon-like activity. The biological behavior of BL-20803 is shown to have striking similarities with that of the structurally different low-molecular-weight inducer tilorone hydrochloride.
BL - 20803是一种低分子量化合物，尽管它能够在小鼠体内引发循环干扰素，但却无法在体外保护培养的细胞系免受干扰素敏感病毒的感染。在给小鼠口服该药物后，对其进行干扰素含量分析的组织中，脾脏和肺中含有的病毒抑制剂量最大。来自此类给药动物的脾细胞在体外培养时，会向培养基中分泌少量干扰素。脾细胞获得产生干扰素能力的时间顺序与完整小鼠体内循环干扰素反应的发展动力学密切相关。当根据脾细胞对塑料表面的粘附或非粘附特性进行分离时，发现大部分干扰素活性与粘附细胞相关。在细胞培养中暴露于BL - 20803时，来自未处理小鼠的粘附细胞以及程度较轻的非粘附细胞会被刺激产生干扰素样活性。结果表明，BL - 20803的生物学行为与结构不同的低分子量诱导剂盐酸替洛隆具有显著相似性。
