Johnson A D, Metzger J F, Spero L
Infect Immun. 1975 Nov;12(5):1206-10. doi: 10.1128/iai.12.5.1206-1210.1975.
Methods for the production and isolation of exfoliative toxin are described. Fermentation conditions were established under which large quantities of the crude material can be produced. Column chromatography methods, including carboxymethyl cellulose and hydroxyapatite, were utilized to purify the toxic protein. The pure toxin had a molecular weight of 26,000 as determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The pure toxin is a simple protein composed of 17 amino acids. Tests for carbohydrate and for alpha- and beta-hemolysin were negative. The mean effective dose of the purified toxin was 0.5 mug per newborn mouse.
描述了剥脱毒素的生产和分离方法。确定了发酵条件，在此条件下可大量生产粗原料。利用包括羧甲基纤维素和羟基磷灰石在内的柱色谱法来纯化有毒蛋白质。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定，纯毒素的分子量为26,000。纯毒素是一种由17个氨基酸组成的简单蛋白质。碳水化合物以及α-和β-溶血素检测均为阴性。纯化毒素对新生小鼠的平均有效剂量为0.5微克。
